High-performance liquid chromatograph hplc system

The Hewlett Packard Series II 1090 Liquid Chromatograph HPLC system is a high-performance instrument used for separating components in a liquid mixture. It utilizes a process called High Performance Liquid Chromatography (HPLC), where a liquid sample is injected into a stream of solvent flowing through a column packed with a separation medium. As the sample components flow through the column, they separate from each other based on their differential migration.

How Does the HPLC Work?

The HPLC process begins with the injection of a liquid sample into a stream of solvent, known as the mobile phase. This mobile phase flows through a column packed with a separation medium, also called the stationary phase. As the sample components flow through the column, they interact with the stationary phase and separate from each other based on their different affinities. The separated components, known as bands, emerge from the column and are carried to one or more detectors.

The detectors in the HPLC system deliver a voltage response as a function of time, creating a chromatogram. Each peak in the chromatogram represents a specific sample constituent, and the time at which it emerges identifies the constituent with respect to a standard. The area under the peak represents the quantity of the constituent.

Instrumentation of Liquid Chromatography

The Hewlett Packard Series II 1090 Liquid Chromatograph HPLC system is equipped with advanced instrumentation designed to optimize the performance of the system. The system includes reciprocating or syringe-type pumps with electronic control to provide accurate and precise flow rates, reproducible solvent mixing, low gradient delay, and efficient mobile phase usage. The dual-piston reciprocating pump is preferred for gradient elution, while the syringe pumps have lower flow pulsation and can be configured using a dual-syringe pump approach for isocratic elution.

The injector devices of the HPLC system are designed to have precision, low dead-volume, and minimal flow disturbance to ensure maximum column efficiency and separation resolution. The system also allows for different injection strategies, such as direct injection, partial loop injection, column switching, and on-column focusing, to increase the injection volume without overloading the column or compromising the separation.

The fittings and unions used to connect the micro-LC modules in the HPLC system are designed to have minimal dead volume, preventing band broadening and ensuring optimal resolution. The system also includes temperature control capabilities for optimizing chromatographic separations, absorbance-based detectors with miniaturized cells to prevent extra band broadening, and various detection techniques such as Laser Induced Fluorescent (LIF) detection, electrochemical detectors (ECDs), and mass spectrometry (MS) for enhanced sensitivity, selectivity, and detectability.

Benefits of the Hewlett Packard Series II 1090 Liquid Chromatograph HPLC System

The Hewlett Packard Series II 1090 Liquid Chromatograph HPLC system offers numerous benefits for users in the field of liquid chromatography. Some of these benefits include:

• High-performance separation of components in a liquid mixture
• Accurate and precise flow rates for reproducible results
• Optimized solvent mixing and low gradient delay
• Efficient mobile phase usage for cost savings
• Various injection strategies for increased injection volume
• Minimal dead volume fittings and unions for optimal resolution
• Temperature control for retention time optimization
• Miniaturized cells for absorbance-based detection
• Advanced detection techniques for enhanced sensitivity and selectivity

What is the purpose of the Hewlett Packard Series II 1090 Liquid Chromatograph HPLC system?

The purpose of the Hewlett Packard Series II 1090 Liquid Chromatograph HPLC system is to separate components in a liquid mixture using the process of High Performance Liquid Chromatography (HPLC). It is commonly used in scientific research, pharmaceutical analysis, and quality control in various industries.

What are the key features of the Hewlett Packard Series II 1090 Liquid Chromatograph HPLC system?

The key features of the Hewlett Packard Series II 1090 Liquid Chromatograph HPLC system include accurate and precise flow rates, optimized solvent mixing, low gradient delay, efficient mobile phase usage, various injection strategies, minimal dead volume fittings, temperature control, miniaturized absorbance-based detection, and advanced detection techniques such as Laser Induced Fluorescent (LIF) detection, electrochemical detectors (ECDs), and mass spectrometry (MS).

How does the HPLC process work?

The HPLC process involves injecting a liquid sample into a stream of solvent flowing through a column packed with a separation medium. As the sample components flow through the column, they separate from each other based on their differential migration. The separated components are then detected and analyzed based on their retention time and peak area in the chromatogram.

What are the advantages of using the Hewlett Packard Series II 1090 Liquid Chromatograph HPLC system?

The advantages of using the Hewlett Packard Series II 1090 Liquid Chromatograph HPLC system include high-performance separation, accurate and precise flow rates, reproducible results, efficient mobile phase usage, increased injection volume without overloading the column, optimal resolution, temperature control for retention time optimization, miniaturized absorbance-based detection, and advanced detection techniques for enhanced sensitivity and selectivity.

In what industries is the Hewlett Packard Series II 1090 Liquid Chromatograph HPLC system commonly used?

The Hewlett Packard Series II 1090 Liquid Chromatograph HPLC system is commonly used in industries such as pharmaceuticals, biotechnology, environmental analysis, food and beverage, forensic science, and research laboratories. It is widely employed for quality control, sample analysis, and research purposes.

The Hewlett Packard Series II 1090 Liquid Chromatograph HPLC system is a high-performance instrument that utilizes the process of High Performance Liquid Chromatography (HPLC) to separate components in a liquid mixture. It offers advanced instrumentation, accurate flow rates, efficient solvent mixing, and various injection strategies for optimal separation and analysis. With its precise detection capabilities and enhanced sensitivity, the HPLC system is widely used in various industries for quality control, research, and analysis purposes.